Optical microscopy is the measurement technique used to view objects, living systems that cannot be seen with unaided eye. Microscopy can be implemented by using various elementary particles. The optical (or photonic) microscope uses photons, while the electron microscope, as its name suggests, is based on electrons.
Optical microscopy physics
The optical microscope is based on converging lenses providing for enlarged images.
To construct an image, the focusing lens captures the wave reflected by a sample causing it to pass through eyepiece. An object is placed in front of the first lens called "objective." The inverted image of different size can be brought about from the object situated beyond the focal length. The second lens is the eyepiece positioned in such a way that the image is situated in focal plane. Thus, the eye observes the image with a better visual comfort. The resulting image is much more than a snapshot of preparation.
Optical microscopy
There is a multitude of microscopy techniques giving access to various types of information.
- Two-photon fluorescence microscopy
- Two-photon microscopy of second harmonic generation
Courtesy of IPCMS
