Fluorescence microscopy is based on the capacity of certain molecules to emit light when illuminated by higher-wavelength light. Basically, captured by the lens, the emitted light is first filtered to isolate interference wavelength that will excite a sample until it is focused by the lens on the observation area.
Photomultiplier-assisted two-photon microscopy is designed to detect fluorescence induced by two-photon absorption. The goal of the technique is to track the in situ evolution of biological species previously marked by endogenous or exogenous fluorophores. The system allows to probe dimensional tissues with a sub-micron resolution.
Shown are laser beam path at 800 nm (red) and fluorescence signal sensing (green)
Tobacco root cell. Image size: 100x100 µm. Resolution: 0.5 μ
Courtesy of IPCMS
